PEPD Mouse Monoclonal Antibody [Clone ID: OTI1B7]

CAT#: CF501727

Carrier-free (BSA/glycerol-free) PEPD mouse monoclonal antibody, clone OTI1B7 (formerly 1B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (RC201970, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD. Positive lysates LY424818 (100ug) and LC424818 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PEPD monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PEPD monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501727, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501727, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501727, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501727, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501727, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501727, Dilution 1:50)

• Anti-PEPD mouse monoclonal antibody (TA501727) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PEPD (RC201970).

• HEK293T cells transfected with either RC201970 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PEPD antibody (TA501727), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1B7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: peptidase D
• Database Link:
  NP_000276
  Entrez Gene 18624 MouseEntrez Gene 292808 RatEntrez Gene 5184 Human
  UniProt ID P12955
• Background: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: PROLIDASE

Reference Data

• Protein Families: Druggable Genome, Protease