DHPS Mouse Monoclonal Antibody [Clone ID: OTI2C9]

CAT#: CF501393

Carrier-free (BSA/glycerol-free) DHPS mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHPS (Cat# RC204235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHPS(Cat# TA501393). Positive lysates LY400714 (100ug) and LC400714 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2C9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DHPS (NP_001921) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.8 kDa
• Gene Name: deoxyhypusine synthase
• Database Link:
  NP_001921
  Entrez Gene 288923 RatEntrez Gene 330817 MouseEntrez Gene 1725 Human
  UniProt ID P49366
• Background: The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity. [provided by RefSeq]
• Synonyms: DHS; DS; MIG13