STAT1 Mouse Monoclonal Antibody [Clone ID: OTI15H3]

CAT#: CF500566

Carrier-free (BSA/glycerol-free) STAT1 mouse monoclonal antibody, clone OTI15H3 (formerly 15H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (RC201075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1. Positive lysates LY408329 (100ug) and LC408329 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-STAT1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500566, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-STAT1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500566, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500566, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-STAT1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500566, Dilution 1:50)

• 

  Anti-STAT1 mouse monoclonal antibody (TA500566) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1 (RC201075).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI15H3
• Applications: IF, WB
• Recommended Dilution: WB 1:2000 IHC 1:50 IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human STAT1 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91 kDa
• Gene Name: signal transducer and activator of transcription 1
• Database Link:
  NP_644671
  Entrez Gene 20846 MouseEntrez Gene 25124 RatEntrez Gene 6772 Human
  UniProt ID P42224
• Background: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
• Synonyms: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer, Toll-like receptor signaling pathway