SIGLEC9 Mouse Monoclonal Antibody [Clone ID: OTI3G8]

CAT#: CF500373

Carrier-free (BSA/glycerol-free) SIGLEC9 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIGLEC9 (RC206674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIGLEC9. Positive lysates LY402335 (100ug) and LC402335 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-SIGLEC9 monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI3G8
• Applications: WB
• Recommended Dilution: WB: 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SIGLEC9 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: sialic acid binding Ig like lectin 9
• Database Link:
  NP_055256
  Entrez Gene 27180 Human
  UniProt ID Q9Y336
• Background: Putative adhesion molecule that mediates sialic-acid dependent binding to cells.Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
• Synonyms: CD329; CDw329; FOAP-9; OBBP-LIKE; siglec-9

Reference Data

• Protein Families: Druggable Genome, Transmembrane