HNF6 (ONECUT1) Mouse Monoclonal Antibody [Clone ID: OTI4F12]

CAT#: CF500196

Carrier-free (BSA/glycerol-free) HNF6 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HNF6 (Cat# RC218219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6(Cat# TA500196). Positive lysates LY401429 (100ug) and LC401429 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500196)

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  Anti-HNF6 mouse monoclonal antibody (TA500196) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6 (RC218219).

Specifications

Product Data

• Clone Name: OTI4F12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.8 kDa
• Gene Name: one cut homeobox 1
• Database Link:
  NP_004489
  Entrez Gene 15379 MouseEntrez Gene 25231 RatEntrez Gene 3175 Human
  UniProt ID Q9UBC0
• Background: The protein encoded by HNF6 is Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a varietyof gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
• Synonyms: HNF-6; HNF6; HNF6A

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young