Sonic Hedgehog (SHH) Mouse Monoclonal Antibody [Clone ID: OTI3A2]

CAT#: CF500040

Carrier-free (BSA/glycerol-free) SHH mouse monoclonal antibody, clone OTI3A2 (formerly 3A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (RC222175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH. Positive lysates LY424868 (100ug) and LC424868 (20ug) can be purchased separately from OriGene.

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  Anti-SHH mouse monoclonal antibody (TA500040) immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH (RC222175).

Specifications

Product Data

• Clone Name: OTI3A2
• Applications: IF, WB
• Recommended Dilution: WB: 1:2000, IF (1:100)
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.6 kDa (Actual 22 kDa/55 kDa)
• Gene Name: sonic hedgehog signaling molecule
• Database Link:
  NP_000184
  Entrez Gene 20423 MouseEntrez Gene 29499 RatEntrez Gene 6469 Human
  UniProt ID Q15465
• Background: SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.
• Synonyms: HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer