Fibronectin (FN1) Rabbit Polyclonal Antibody

CAT#: BP8036

Fibronectin (FN1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: Immunofluorescence: 1/80.
  Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8°C over night.
  ELISA (> / = 1/200).
  RIA.
  Positive Control: Human skin.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified fibronectin from human plasma
• Specificity: This antibody is specific for Fibronectin (100%).
  Collagen type I, III, IV and VI < 0.1% (RIA at 1/10000 dilution).
  This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
• Formulation: Phosphate buffered solution without BSA or preservatives.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fibronectin 1
• Database Link:
  Entrez Gene 2335 Human
  UniProt ID P02751
• Background: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
• Synonyms: FN1, Cold-insoluble globulin, CIG