Col1a1 Rabbit Polyclonal Antibody

CAT#: BP8002S

Col1a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA
  RIA: 1/200 (OD ≥ 500).
  Immunofluorescence : ≥ 1/40.
  Immunohistochemistry on Paraffin Sections: 1/200-1/600.
  Incubation Time: 60 min at RT or 2-8°C over night.
  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Positive Control: Rat skin or liver.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type I from Rat skin
• Specificity: Rat Collagen type I: 100%
  Rat Collagen II, III and V: < 0.1%
  Human, Mouse, Chicken Collagen type I: < 0.1%
  Rat Elastin: < 0.1% (Determined by RIA at 1/200 dilution).
• Formulation: PBS
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 0.1 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen, type I, alpha 1
• Database Link:
  Entrez Gene 29393 Rat
  UniProt ID P02454
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I collagen (95kDa) is mainly found in bone, cornea, skin and tendon.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen