Integrin beta 1 (ITGB1) (beta-1D Isoform) Mouse Monoclonal Antibody [Clone ID: 2B1]

CAT#: BM6028P

Integrin beta 1 (ITGB1) (beta-1D Isoform) mouse monoclonal antibody, clone 2B1, Purified

Product Images

Frozen Sections of Porcine myocardium immunostained for integrin b1D using a 1/200 dillution of 2B1

• 

  Negative Control: only secondary antibody applied.

Specifications

Product Data

• Clone Name: 2B1
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/100-1/1000
  Immunocytochemistry.
  Flow Cytometry.
  Immunohistochemistry on Frozen Tissues: 1/25 -1/200 with ABC as detection reagent.
• Reactivities: Canine, Human, Mouse, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to the C-terminal 24 amino acids of integrin b1D including an appending N-terminal cysteine coupled to keyhole limpet hemocyanin.
• Specificity: 2B1 recognizes specifically the cytoplasmic domain of integrin subunit b1D present in cardiac and skeletal muscle.
• Formulation: PBS with 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 1
• Database Link:
  Entrez Gene 3688 Human
  UniProt ID P05556
• Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. bea1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12