Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: RGE53]

CAT#: BM6005P

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone RGE53, Purified

Product Images

Immunohistochemistry on frozen section of human kidney epithelium

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  Immunohistochemistry on frozen section of human colon

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  Immunohistochemistry on frozen section of swine liver hepatocytes

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  Immunofluorescence staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining

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  Immunofluorescence staining pattern of internal epithelia as well as epidermis of 2 days old zebrafish embryo

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  Immunofluorescence staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining

• Immunofluorescence staining of 1 month old zebrafish embryo

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Specifications

Product Data

• Clone Name: RGE53
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/100-1/1000.
  Immunocytochemistry.
  Flow Cytometry.
  Immunohistochemistry on Frozen tissues: 1/100-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
• Reactivities: Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeletal preparation of cells.
• Specificity: Clone RGE53 reacts exclusively with cytokeratin 18 which is present in glandular epithel cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: keratin 18
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Cytokeratins are a subfamily of intermediatefilament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
  Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18