Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.27]

CAT#: BM5503P

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.27, Purified

Specifications

Product Data

• Clone Name: Ks18.27
• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: 1:10; 1 h at RT.
  Cytological material.
  Immunoblotting.
  ELISA.
• Reactivities: Bovine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeletal preparation from tumor cell line MCF-7.
• Specificity: Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18; formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine; negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA.
• Formulation: PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5% BSA
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore in 1 ml dist. water
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 18
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18