Synaptopodin (SYNPO) Mouse Monoclonal Antibody [Clone ID: G1D4]
Synaptopodin (SYNPO) mouse monoclonal antibody, clone G1D4, Aff - Purified
|Applications||IF, IHC, WB|
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections (After microwave treatment).
Working Dilutions: 1/5-1/10 for 1h at RT.
Western blot: 0.1-0.5 µg/ml.
The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
|Reactivity||Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat|
|Immunogen||Isolated Rat kidney glomeruli.|
|Specificity||The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells.
Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.
Negative Species: Rabbit, Frog and Chicken.
|Formulation||Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
State: Aff - Purified
State: Lyophilized purified Ig fraction
|Reconstitution Method||Restore with 1 ml distilled water|
|Purification||Affinity Chromatography on Protein A|
Prior to reconstitution store at 2-8°C.
|Stability||Shelf life: one year from despatch.|
|Background||Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).|