Synaptopodin (SYNPO) Mouse Monoclonal Antibody [Clone ID: G1D4]

CAT#: BM5086P

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Synaptopodin (SYNPO) mouse monoclonal antibody, clone G1D4, Aff - Purified



USD 490.00


Availability*
2 Weeks

Size
    • 50 ug


Product images

Specifications

Product Data
Clone Name G1D4
Applications IF, IHC, WB
Recommended Dilution Immunofluorescence. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections (After microwave treatment). 
Working Dilutions: 1/5-1/10 for 1h at RT. 
Western blot: 0.1-0.5 µg/ml. 
The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Reactivity Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Isolated Rat kidney glomeruli.
Specificity The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. 
Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.
Negative Species: Rabbit, Frog and Chicken.
Formulation Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
State: Aff - Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 1 ml distilled water
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer. 
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Gene Name synaptopodin
Background Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Synonyms KIAA1029
Reference Data
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