Synaptopodin (SYNPO) Mouse Monoclonal Antibody [Clone ID: G1D4]

CAT#: BM5086P

Synaptopodin (SYNPO) mouse monoclonal antibody, clone G1D4, Aff - Purified

Product Images

Western blotting for Synaptopodin on Human kidney lysate using Acris anti-SYNPO monoclonal antibody. The antibody reacts with a band of approximate size 44kDa that is thought to result from the breakdown of the full length protein.

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  Staining for Synaptopodin on formalin fixed paraffin embedded kidney tissue sections using anti-SYNPO monoclonal antibody following antigen retrieval. Indirect immunoperoxidase staining. DAB substrate, hematoxylene counterstain.

Specifications

Product Data

• Clone Name: G1D4
• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (After microwave treatment).
  Working Dilutions: 1/5-1/10 for 1h at RT.
  Western blot: 0.1-0.5 µg/ml.
  The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
• Reactivities: Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Isolated Rat kidney glomeruli.
• Specificity: The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells.
  Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.
  Negative Species: Rabbit, Frog and Chicken.
• Formulation: Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: synaptopodin
• Database Link:
  Entrez Gene 11346 Human
  UniProt ID Q8N3V7
• Background: Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
• Synonyms: KIAA1029