SCF (KITLG) Mouse Monoclonal Antibody [Clone ID: hKL12]

CAT#: BM4117

SCF (KITLG) mouse monoclonal antibody, clone hKL12, Purified

Specifications

Product Data

• Clone Name: hKL12
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 0.15 µg/ml as a titer in ELISA against the recombinant antigen.
  Has been described to work in Wetsern blots and Immunohistochemistry.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human stem cell factor.
• Specificity: This Monoclonal antibody hKL12 is specific for Stem Cell Factor (hSCF). hKL12 is useful for the detection of hSCF in tissues, isolated cells and for studying biological effects of Human Stem Cell Factor in vitro.
  Antigen distribution on Tissue sections: Bone marrow stromal cells, fibroblasts, and fetal liver cells express SCF. Keratinocytes stain positive.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: KIT ligand
• Database Link:
  Entrez Gene 4254 Human
  UniProt ID P21583
• Background: SCF is a transmembrane protein expressed as two alternatively spliced isoforms, either 220 or 248 amino acids, the former lacking a peptidase cleavage site. The cleavage site is responsible for the release of the soluble form of the protein from the cell surface. The first 164 or 165 residues of the extracellular domain comprise soluble SCF, which exists in solution as a noncovalently linked homodimer. Alone, SCF is a weakly proliferative stimulus but acts synergistically with numerous cytokines. SCF is important in germ cell developement, and an indispensable facor for mast cell proliferation an differentiation.
• Synonyms: KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer