Adgre1 Rat Monoclonal Antibody [Clone ID: CI:A3-1]

CAT#: BM4008FS

Adgre1 rat monoclonal antibody, clone CI:A3-1, FITC

Product Images

Mouse anti-Macrophage F4/80 antigen antibody (5 ug/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.

• 

  Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN antibody -FITC

• 

  Mouse anti-Macrophage F4/80 antigen antibody on thioglycollate elicited mouse peritoneal macrophages. Purple: ; Green: Isotype control SM19FS; Percentages reflect % positive after subtraction of negative control, using M1 marker.

Specifications

Product Data

• Clone Name: CI:A3-1
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry.
  Immunofluorescence.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 myeloma cell line.
• Specificity: This antibody recognises the Murine F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. We recommend the use of antibody BM4008A for this purpose
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: adhesion G protein-coupled receptor E1
• Database Link:
  Entrez Gene 13733 Mouse
  UniProt ID Q61549
• Background: F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins which shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
• Synonyms: Emr1, Gpf480