TIA1 Mouse Monoclonal Antibody [Clone ID: 2G9A10F5]
TIA1 mouse monoclonal antibody, clone 2G9A10F5, Purified
|Recommended Dilution||Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval is recommended.
Use Polymer anti Mouse/Rabbit IgG as a detection system.
Positive Control: Tonsil.
|Immunogen||Human bone marrow malignant cells from a non-B, non-T acute leukemia.|
|Specificity||Within hematopoietic cell lines, the 'TIA-1' monoclonal antibody (2G9) reacts with about 90% of CD16+, 50-60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. (5) It reacts with almost all monocytes and granulocytes. (3) This antibody also reacts with CD4+ activated T-cell clones, activated NK cell clones, and Con A-activated thymocytes, but not with B lymphocytes or B-cell lines. (1,5)
The 2G9A10F5 monoclonal antibody was evaluated during the 5th International Workshop on Human Leucocyte Differentiation Antigens, in Boston, USA, in the section of monoclonal antibodies reactive with intracellular antigens. (6)
Cellular Localization: Cytoplasmic.
Buffer: Tris‐HCI containing stabilizing protein and <0.1% sodium azide.
|Purification||Protein G Chromatography|
|Storage||Store the antibody undiluted at 2-8°C.|
|Stability||Shelf life: one year from despatch.|
|Gene Name||Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1|
|Background||TIA1 is a member of an RNA binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 17-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.|
|Synonyms||Nucleolysin TIA-1, p40-TIA-1, p15-TIA-1|
|Protein Families||Druggable Genome|
|Protein Pathways||Mismatch repair|
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