CD24 Mouse Monoclonal Antibody [Clone ID: ALB9]

CAT#: BM2357

CD24 mouse monoclonal antibody, clone ALB9, Azide Free

Specifications

Product Data

• Clone Name: ALB9
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 2 µg/5x10e5cells/test.
  Immunohistochemistry on frozen sections or cell smears (1:25-1:50 dilution).
  Studies of peripheral blood B cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human bone marrow malignant cells from a non-B, non-T acute leukemia
• Specificity: This antibody recongnizes CD24. The molecular weight of the recognized antigen is 35-45 kDa.
  Recognizes B cells from pre-B to mature-B stage.
  The ALB9 monoclonal antibody has been assigned to the CD24 cluster of differentiation at the sixth International Workshop on Human Leucocyte Differentiation Antigens held in Kobe, Japan in 1996.
• Formulation: PBS containing 1 mg/ml BSA as stabilizer, containing no preservatives
  State: Azide Free
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1 ml of distilled water.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the reconstituted antibody at 2-8°C for one month (add 0.09% Sodium Azide) or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD24 molecule
• Database Link:
  Entrez Gene 100133941 Human
  UniProt ID P25063
• Background: CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer.
• Synonyms: X62 heat stable antigen, HSA