GFAP Mouse Monoclonal Antibody [Clone ID: GF12.24]

CAT#: BM2287

GFAP mouse monoclonal antibody, clone GF12.24, Purified

Specifications

Product Data

• Clone Name: GF12.24
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunofluorescence microscopy / Immunohistochemistry on cytological material.
  Immunohistochemistry on frozen sections: 1/10.
  Immunohistochemistry on paraffin-embedded sections: 1/10 (microwave treatment recommended).
  
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Intermediate filament cytoskeleton from cultured human glioma cells.
• Specificity: GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects.
  Polypeptide Reacting: Mr 50 000 glial filament protein GFP.
  Tumors Specifically Reacting: Astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas.
  Tested Reactivities on Cultured Cell Lines: U 333 CG/343MG.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1.0 ml distilled water
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes
• Synonyms: Glial Fibrillary Acidic Protein