EPCAM Mouse Monoclonal Antibody [Clone ID: HEA125]

CAT#: BM2274X

EPCAM mouse monoclonal antibody, clone HEA125, Purified

Specifications

Product Data

• Clone Name: HEA125
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: Use 5 µl of neat antibody to label 10⁶ cells or 100 µl cell suspension.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections and Paraffin Sections: 1/20.
  This product requires protein digestion pre-treatment of paraffin sections e.g. 0.1% pronase, 10 minutes.
  Recommended Positive Control: Human colon and appendix.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HT29 carcinoma cell line.
  Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
• Specificity: This antibody recognizes the 34kDa cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM).
• Formulation: PBS, pH 7.3
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: epithelial cell adhesion molecule
• Database Link:
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
• Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18