beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B1G6]

CAT#: BM2145

beta 2 Microglobulin (B2M) mouse monoclonal antibody, clone B1G6, Purified

Specifications

Product Data

• Clone Name: B1G6
• Applications: FC, IF, IHC
• Recommended Dilution: Purification of the HLA antigens associated with Beta 2-Microglobulin.
  Fluorescence Microscopy or Flow Cytometry: 2 µl/5x10⁵ cells/test.
  Immunohistochemistry on Frozen Sections or Cell Smears: Working dilution 1/25-1/50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified human Beta 2-Microglobulin
• Specificity: Specific for Human beta 2-Microglobulin (MW 12 kDa).
  Recognizes the HLA-beta 2-Microglobulin complex, free or associated with the membrane. Does not react with other species.
  The exact epitope for the antibody has not been mapped.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 1 mg/ml BSA
  Preservative: None
• Reconstitution Method: Restore with 1.0 ml distilled water
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Addition of 0.09% (w/v) Sodium Azide is recommended for storage up to one month at 2-8°C.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: beta-2-microglobulin
• Database Link:
  Entrez Gene 567 Human
  UniProt ID P61769
• Background: Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin