beta III Tubulin (TUBB3) Mouse Monoclonal Antibody [Clone ID: TU-20]

CAT#: BM170S

beta III Tubulin (TUBB3) mouse monoclonal antibody, clone TU-20, Purified

Product Images

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 ug/ml). Nuclei were stained with DAPI (blue).

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  Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B - Merged image of co-staining with anti-beta-tubulin (TU-06; green). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

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  Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B - Merged image of co-staining with anti-beta-tubulin (TU-06; green). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

Specifications

Product Data

• Clone Name: TU-20
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 1-2 µg/ml, 90 min (Reducing conditions).
  Positive Control: Porcine brain lysate.
  Negative Control: HPB-ALL peripheral blood leukemia cell line.
  Sample Preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Staining Technique: Standard ABC technique (DAB+).
  Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT or high temperature citrate buffer antigen retrieval.
  Positive tissue: Neuronal tissue.
  Immunocytochemistry.
  Positive Material: Neuro2a mouse neuroblastoma cell line.
• Reactivities: Broad
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide (C) 441-448 coupled to Maleimide-activated Keyhole Limpet Hemocyanin via Cysteine added to the N-terminus of the neuron-specific peptide.
• Specificity: This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of Human class III β-tubulin specific for neurones.
  The antibody is a highly specific marker for neuronal tissue.
  TU-20 is very useful for the detection of microtubule structures on fixed cells.
  This Monoclonal antibody TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate and Caprylic Acid Precipitation
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: tubulin beta 3 class III
• Database Link:
  UniProt ID Q13509
• Background: The beta-III Tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
• Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4