Aurora B (AURKB) pThr232 Rabbit Polyclonal Antibody

CAT#: AP55931PU-N

Aurora B (AURKB) pThr232 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) antibody.The lane on the right is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:500~1:1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 232 (R-K-T(p)-M-C) derived from Human AurB (KLH-conjugated)
• Specificity: The antibody detects endogenous level of AurB only when phosphorylated at threonine 232.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 39 kDa
• Gene Name: aurora kinase B
• Database Link:
  Entrez Gene 9212 Human
  UniProt ID Q96GD4
• Background: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.
• Synonyms: Aurora-B, Aurora/IPL1-related kinase 2, STK-1, AURKB, AIK2, AIM1, ARK2, STK12