IKK gamma (IKBKG) pSer85 Rabbit Polyclonal Antibody

CAT#: AP55883PU-N

IKK gamma (IKBKG) pSer85 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-IKK-gamma (Ser85) antibody.The lane on the right is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:500~1:1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 85 (Q-A-S(p)-Q-R) derived from Human IKK-gamma (KLH-conjugated)
• Specificity: The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 48 kDa
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Database Link:
  Entrez Gene 8517 Human
  UniProt ID Q9Y6K9
• Background: Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.
• Synonyms: FIP3, FIP-3, IKKAP1, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma