CEBP Alpha (CEBPA) pThr230 Rabbit Polyclonal Antibody

CAT#: AP55806PU-N

CEBP Alpha (CEBPA) pThr230 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from cos-7 cells (Lane 2), using C/EBP-a (Phospho-Thr230) Antibody. The lane on the left is treated with antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:500~1:1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 230 (P-P-T(p)-P-V) derived from Human C/EBP-α (KLH-conjugated)
• Specificity: The Antibody detects endogenous levels of C/EBP-α only when phosphorylated at Thr230.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42 kDa
• Gene Name: CCAAT/enhancer binding protein alpha
• Database Link:
  Entrez Gene 1050 Human
  UniProt ID P49715
• Background: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
• Synonyms: C/EBP alpha