CD22 pTyr807 Rabbit Polyclonal Antibody

CAT#: AP55784PU-S

CD22 pTyr807 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from COS7 cells (Lane 2), using BL-CAM (Phospho-Tyr807) Antibody.The lane on the left is treated with antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:500~1:1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 807 (G-D-Y(p)-E-N) derived from Human BL-CAM (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of BL-CAM only when phosphorylated at tyrosine 807.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 95 kDa
• Gene Name: CD22 molecule
• Database Link:
  Entrez Gene 933 Human
  UniProt ID P20273
• Background: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
• Synonyms: SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM