Estrogen Receptor 1 (ESR1) pTyr537 Rabbit Polyclonal Antibody

CAT#: AP55697PU-S

Estrogen Receptor 1 (ESR1) pTyr537 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-a (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-a (Phospho-Tyr537) Antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 537(P-L-Y(p)-D-L) derived from Human Estrogen Receptor-α (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of ER-α only when phosphorylated at tyrosine 537.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66 kDa
• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1