CEBP Alpha (CEBPA) pSer21 Rabbit Polyclonal Antibody

CAT#: AP55691PU-N

CEBP Alpha (CEBPA) pSer21 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-a (phospho-Ser21) Antibody.The lane on the right is treated with the antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-a (phospho-Ser21) antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45 kDa
• Gene Name: CCAAT/enhancer binding protein alpha
• Database Link:
  Entrez Gene 1050 Human
  UniProt ID P49715
• Background: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
• Synonyms: C/EBP alpha