PADI4 Rabbit Polyclonal Antibody

CAT#: AP55150SU-N

PADI4 rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/1000-1/5000.
  Western Blot: 1/200-1/2000.
  Immunoprecipitation: 1/20-1/100.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A15 residue synthetic peptide derived from C-terminal domain of Human PADI4 protein
• Specificity: Reacts with 74 kDa PAD4 protein from Human, Mouse and Rat.
  No cross reaction with the other isoforms.
• Formulation: State: Serum
  State: Lyophilized powder
  Preservative: None
• Reconstitution Method: Restore in 0.2 ml distilled water.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: peptidyl arginine deiminase 4
• Database Link:
  Entrez Gene 23569 Human
  UniProt ID Q9UM07
• Background: PAD4 is a member of a family of enzymes responsible for the conversion of arginine residues to citrulline residues via catalyzation of the deimination of the arginine residues. PAD4 down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. This protein may play a role in granulocyte and macrophage development leading to inflammation and immune response.
• Synonyms: PDI4, PAD4, PDI5, PADI5, PADI-H, HL-60 PAD