TAF1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP54141PU-N

TAF1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

TAF1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TAF1 antibody detected the TAF1 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1860-1893 amino acids from the C-terminal region of human TAF1
• Specificity: This antibody reacts to TAF1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 106kd Isoform 2. 212677 Da
• Gene Name: TATA-box binding protein associated factor 1
• Database Link:
  Entrez Gene 270627 MouseEntrez Gene 6872 Human
  UniProt ID P21675
• Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.
• Synonyms: BA2R, CCG1, CCGS, TAFII250

Reference Data

• Protein Families: Protein Kinase
• Protein Pathways: Basal transcription factors