PDE3B (Center) Rabbit Polyclonal Antibody

CAT#: AP53235PU-N

PDE3B (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PDE3B Antibody (Center) in Jurkat cell line lysates (35ug/lane). This demonstrates the PDE3B antibody detected the PDE3B protein (arrow).

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  Confocal immunofluorescent analysis of PDE3B Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of Jurkat cells using PDE3B Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution:

  Western Blot: 1/500 - 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/10.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 400-427 amino acids from the Central region of Human PDE3B
• Specificity: This antibody recognizes Human PDE3B (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: phosphodiesterase 3B
• Database Link:
  Entrez Gene 5140 Human
  UniProt ID Q13370
• Background: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes; PDE3B may play a role in fat metabolism.
• Synonyms: Phosphodiesterase 3B, Phosphodiesterase-3B cGMP-inhibited, HcGIP1, CGIPDE1, CGI-PDE B, CGIP, CGIP1
• Note: Molecular Weight: 124333 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism