NUMB (N-term) Rabbit Polyclonal Antibody

CAT#: AP52967PU-N

NUMB (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of NUMB Antibody (N-term) in NCI-H292 cell line lysates (35ug/lane). This demonstrates the NUMB antibody detected the NUMB protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue reacted with NUMB Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NUMB
• Specificity: This antibody recognizes Human NUMB (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: NUMB, endocytic adaptor protein
• Database Link:
  Entrez Gene 8650 Human
  UniProt ID P49757
• Background: The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Protein S171, h-Numb
• Note: Molecular Weight: 70804 Da

Reference Data

• Protein Pathways: Notch signaling pathway