Integrin alpha 5 (ITGA5) (Center) Rabbit Polyclonal Antibody

CAT#: AP52251PU-N

Integrin alpha 5 (ITGA5) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ITGA5 (arrow) using CD49e / ITGA5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ITGA5 gene.

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  Western blot analysis of CD49e / ITGA5 Antibody (Center) in human uterine tumor and mouse bladder tissue lysates (35ug/lane). This demonstrates the ITGA5 antibody detected the ITGA5 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with CD49e / ITGA5 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 574-602 amino acids from the Central region of Human CD49e / ITGA5
• Specificity: This antibody recognizes Human CD49e / ITGA5 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: integrin subunit alpha 5
• Database Link:
  Entrez Gene 3678 Human
  UniProt ID P08648
• Background: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA
• Note: Molecular Weight: 114536 Da

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton