GSTA3 (N-term) Rabbit Polyclonal Antibody

CAT#: AP51965PU-N

GSTA3 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GSTA3 Antibody (N-term) in NCI-H292 cell line lysates (35ug/lane). This demonstrates the GSTA3 antibody detected the GSTA3 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GSTA3.
• Specificity: This antibody recognizes Human GSTA3 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: glutathione S-transferase alpha 3
• Database Link:
  Entrez Gene 2940 Human
  UniProt ID Q16772
• Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined.
• Synonyms: Glutathione S-transferase A3-3
• Note: Molecular Weight: 25302 Da

Reference Data

• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450