FAT10 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51613PU-N

FAT10 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of FAT10 Antibody (C-term) Cat.-No AP51613PU-Nin mouse liver tissue lysates (35ug/lane). This demonstrates the hFAT10 antibody detected the hFAT10 protein (arrow).

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  Formalin fixed and paraffin embedded human stomach carcinoma reacted with FAT10 Antibody (C-term) Cat.-NoAP51613PU-Nfollowed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of FAT10 Antibody (C-term) Cat.-NoAP51613PU-Nwith HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: approx.1/1000.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human FAT10
• Specificity: This antibody recognizes Human and Mouse FAT10 (C-term).
  Cellular Location:
  Nucleus. Cytoplasm Note - Accumulates in aggresomes under proteasome inhibtion conditions.
  Tissue Location:
  Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the RES (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  UniProt ID AAD52982
• Synonyms: UBD
• Note: Molecular Weight: 18;473 Da