eNOS (NOS3) Rabbit Polyclonal Antibody

CAT#: AP51430PU-N

eNOS (NOS3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of NOS3 Antibody in K562 cell line lysates (35ug/lane). This demonstrates the eNos antibody detected the eNos protein (arrow).

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  Flow cytometric analysis of K562 cells using NOS3 Antibody (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1163-1191 amino acids from Human eNOS
• Specificity: This antibody recognizes Human eNOS (1163-1191).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: nitric oxide synthase 3
• Database Link:
  Entrez Gene 4846 Human
  UniProt ID P29474
• Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS
• Note: Molecular Weight: 133289 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway