DDX27 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51219PU-N

DDX27 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DDX27 Antibody (C-term) in K562 cell line lysates (35ug/lane). This demonstrates the DDX27 antibody detected the DDX27 protein (arrow).

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  Formalin fixed and paraffin embedded human prsotate carcinoma stained with DDX27 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining

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  Flow cytometric analysis of K562 cells using DDX27 Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 777-796 amino acids from the C-terminal region of Human DDX27
• Specificity: This antibody recognizes Human DEAD box protein 27 (C-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: DEAD-box helicase 27
• Database Link:
  Entrez Gene 55661 Human
  UniProt ID Q96GQ7
• Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].
• Synonyms: RHLP, HSPC259, PP3241, DEAD box protein 27
• Note: Molecular Weight: 89835 Da