beta Crystallin A3 (CRYBA1) (Center) Rabbit Polyclonal Antibody

CAT#: AP51091PU-N

beta Crystallin A3 (CRYBA1) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Beta-crystallin A3 Antibody in Mouse heart tissue lysates (35ug/lane).This demonstrates the CRYBA1 antibody detected the CRYBA1 protein (arrow).

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  Western blot analysis of Beta-crystallin A3 Antibody in human placenta tissue lysates (35ug/lane). This demonstrates the CRYBA1 antibody detected the CRYBA1 protein (arrow).

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  Formalin fixed, paraffin embedded human cerebellum tissue stained with Beta-crystallin A3 Antibody followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000
  Western blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 111-141 amino acids from the Central region of Human CRYBA1.
• Specificity: This antibody recognizes Human and Mouse Beta-crystallin A3.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: crystallin beta A1
• Database Link:
  Entrez Gene 12957 MouseEntrez Gene 1411 Human
  UniProt ID P05813
• Background: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'
• Synonyms: CRYBA1, CRYB1
• Note: Molecular Weight: 25150 Da