Ataxin 3 (ATXN3) (Center) Rabbit Polyclonal Antibody

CAT#: AP50312PU-N

Ataxin 3 (ATXN3) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ATXN3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). ATXN3 (arrow) was detected using the purified Pab.

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  Western blot analysis of ATXN3 (arrow) using rabbit polyclonal ATXN3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATXN3 gene.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with ATXN3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  ATXN3 Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow cytometric: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 267~297 amino acids from the Center region of human ATXN3
• Specificity: This antibody reacts to Ataxin-3.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ataxin 3
• Database Link:
  Entrez Gene 4287 Human
  UniProt ID P54252
• Background: ATXN3 was known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers.
• Synonyms: ATXN3, ATX3, MJD, MJD1, SCA3
• Note: Molecular Weight: 41781 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors