S adenosylhomocysteine hydrolase (AHCY) (N-term) Rabbit Polyclonal Antibody

CAT#: AP50117PU-N

S adenosylhomocysteine hydrolase (AHCY) (N-term) rabbit polyclonal antibody, Purified

Product Images

AHCY Antibody (N-term) western blot analysis in mouse liver tissue lysates (35 ug/lane).This demonstrates the AHCY antibody detected the AHCY protein (arrow).

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  AHCY Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AHCY Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  AHCY Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 86-118 amino acids from the N-terminal region of human AHCY
• Specificity: This antibody reacts to AdoHcyase.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: adenosylhomocysteinase
• Database Link:
  Entrez Gene 269378 MouseEntrez Gene 191 Human
  UniProt ID P23526
• Background: S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Adenosylhomocysteinase, SAHH
• Note: Molecular Weight: 47716 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism