Acrosin (ACR) (Center) Rabbit Polyclonal Antibody

CAT#: AP50058PU-N

Acrosin (ACR) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of ACR Antibody (Center) in T47D cell line lysates (35 ug/lane). ACR (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with ACR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  ACR Antibody (Center) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry 1/50 - 1/100.
  Flow cytometric 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 106~135 amino acids from the Center region of human ACR
• Specificity: This antibody reacts to Acrosin.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: acrosin
• Database Link:
  Entrez Gene 49 Human
  UniProt ID P10323
• Background: ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
• Synonyms: acrosin; ACRS; preproacrosin; proacrosin
• Note: Molecular Weight: 45847 Da

Reference Data

• Protein Families: Druggable Genome, Protease