TNF alpha (TNF) Goat Polyclonal Antibody

CAT#: AP33336PU-N

TNF alpha (TNF) goat polyclonal antibody, Purified

Product Images

Human breast invasive ductal carcinoma stained with TNF-alpha Antibody Cat.-No AP33336PU

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  Sandwich ELISA using TNF-alpha Antibody Cat.-No AP33336PU

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Sandwich ELISA: To detect Human TNF-alpha by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha (Cat.-No AP33336BT) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha.
  Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-α (0.25 ng/ml), a concentration of 0.012-0.014 µg/ml of this antibody is required.
  Immunohistochemistry on Parrafin Sections: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 μg/ml-5.0 μg/ml with a two-hour incubation at room temperature or an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived recombinant Human TNF-alpha (Cat.-No PA132)
• Specificity: This antibody recognizes Human TNF-alpha. Other species not tested.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Human TNF-alpha matrix.
  
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin