Desmin (DES) Rabbit Polyclonal Antibody

CAT#: AP33200SU-N

Desmin (DES) rabbit polyclonal antibody, Serum

Product Images

Immunofluorescence staining of a 9 days old Zebrafish embryo.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/100–1/500.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Sections.
  Rrecommended Dilutions: 1/25-1/200 for immunohistochemistry with avidin biotinylated horseradish peroxidase complex (ABC) as detection reagent.
• Reactivities: Bovine, Canine, Chicken, Goat, Hamster, Human, Mouse, Rat, Zebrafish
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Chicken gizzard muscle Desmin.
  Desmin was purified from a crude tissue preparation by preparative gel electrophoresis as described in Reference 1.
• Specificity: This Desmin K5 polyclonal antibody reacts exclusively with Desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
• Formulation: State: Serum
  State: Liquid Serum
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: desmin
• Database Link:
  Entrez Gene 1674 Human
  UniProt ID P17661
• Background: Desmin (53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous.
  Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas.
  Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
• Synonyms: DES