NUMBL (N-term) Goat Polyclonal Antibody

CAT#: AP32925PU-N

NUMBL (N-term) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Numbl with C-terminal MYC tag probed with (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western Blot: 0.5-1 µg/ml. In transfected HEK293 transiently expressing Human Numbl and DYKDDDDK tagged), a band of approx. 85kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody.
  
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the N-terminus of the protein sequence according to NP_004747.1
• Specificity: This antibody recognizes NUMBL.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Slphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: NUMB like, endocytic adaptor protein
• Database Link:
  Entrez Gene 9253 Human
  UniProt ID Q9Y6R0
• Synonyms: Numb-R, Numb-related protein