Metabotropic Glutamate Receptor 5 (GRM5) Chicken Polyclonal Antibody

CAT#: AP32843PU-N

Metabotropic Glutamate Receptor 5 (GRM5) chicken polyclonal antibody, Purified

Product Images

mGluR5 staining of pyramidal and granule cell neurons in the hippocampal formation of an adult transgenic Mouse brain (red satining). Green staining is GFP-immunoreactivity under the control of a constitutive actin promoter

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at 1/2000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody.
  Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgY fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: glutamate metabotropic receptor 5
• Database Link:
  Entrez Gene 2915 Human
  UniProt ID P41594
• Synonyms: Metabotropic glutamate receptor 5, GPRC1E, MGLUR5