mGluR1a / GRM1a Chicken Polyclonal Antibody

CAT#: AP32840PU-N

mGluR1a / GRM1a chicken polyclonal antibody, Purified

Product Images

mGluR1 staining of corticalons inan adult transgenic Mouse brain (red satining). Green staining isGFP-immunoreactivity for a subpopulation of cortical neurons.

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at 1/2000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody.
  Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgY fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: glutamate metabotropic receptor 1
• Database Link:
  Entrez Gene 2911 Human
  UniProt ID Q13255
• Synonyms: Metabotropic glutamate receptor 1, Gprc1a, Mglur1