PTCH1 (266-280) Goat Polyclonal Antibody

CAT#: AP32805PU-N

PTCH1 (266-280) goat polyclonal antibody, Aff - Purified

Product Images

Staining of Mouse fetal Kidney lysate using PTCH1 Antibody at 2 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western blot: 1-3 μg/ml. Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1).
  Immunofluorescence: 5 µg/ml. Strong expression of the protein seen in the cytoplasm of HeLa cells.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence (266-280) according to NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1).
  Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: patched 1
• Database Link:
  Entrez Gene 5727 Human
  UniProt ID Q13635
• Synonyms: PTCH, PTC1, PTC, Patched 1