IL33 Rabbit Polyclonal Antibody

CAT#: AP32086PU-N

IL33 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot unreduced using Interleukin-33 antibody Cat.-No AP32086PU

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  Western Blot reduced using Interleukin-33 antibody Cat.-No AP32086PU

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  Sandwich ELISA using Interleukin-33 antibody Cat.-No AP32086PU

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  Formalin-Fixed, Paraffin-Embedded Sections of human breast malignant ductal adenocarcinoma stained with Interleukin-33 antibody Cat.-No AP32086PU

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution:

  Sandwich ELISA: To detect Human IL-33 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 antibody (Cat.-No AP32086BT-S or AP32086BT-N) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-33.
  Western Blot: To detect Human use this antibody at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Neutralization: To yield one-half maximal inhibition [ND₅₀] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.
  Immunohistochemistry on Paraffin Sections: 0.125-0.25 μg/ml with overnight incubation at 4°C. Staining of Formalin-Fixed, Paraffin Embedded Sections of Human breast malignant ductal adenocarcinoma using an HRP labeled polymer detection system with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human Interleukin 33 (Cat.-No AR20007PU)
• Specificity:

  This antibody detects Human Interleukin-33.
  Other species not tested.

• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized hIL-33 matrix
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: interleukin 33
• Database Link:
  Entrez Gene 90865 Human
  UniProt ID O95760
• Background: Interleukin 33 (IL-33) is a 32 kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T-cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12 kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
• Synonyms: IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26