IL33 Rabbit Polyclonal Antibody

CAT#: AP32086BT-S

IL33 rabbit polyclonal antibody, Biotin

Product Images

Western Blot unreduced using Interleukin-33 antibody Cat.-No AP32086BT

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  Western Blot reduced using Interleukin-33 antibody Cat.-No AP32086BT

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  Direct ELISA using Interleukin-33 antibody Cat.-No AP32086BT

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  Sandwich ELISA using Interleukin-33 antibody Cat.-No AP32086BT

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human IL-33 by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant Human IL-33.
  Sandwich ELISA: To detect Human IL-33 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjuagted antibody, in conjunction with Anti-Human IL-33 antibody (Cat.-No AP32086PU-S or AP32086PU-N) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-33.
  Western Blot: To detect Human IL33 use this antibody at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human Interleukin 33 (Cat.-No AR20007PU)
• Specificity: This antibody detects Human Interleukin-33.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: interleukin 33
• Database Link:
  Entrez Gene 90865 Human
  UniProt ID O95760
• Background: Interleukin 33 (IL-33) is a 32 kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T-cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12 kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
• Synonyms: IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26