APOBEC3A_B Goat Polyclonal Antibody

CAT#: AP31973PU-N

APOBEC3A_B goat polyclonal antibody, Aff - Purified

Product Images

Paraffin Embedded Human Tonsil stained with APOBEC3A antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution:
  Peptide ELISA: 1/8000 (Detection limit).
  Western blot: 3-6 µg/ml. Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.
  Immunohistochemitry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells.
  Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus of A431 and A549 cells.
  Flow Cytometry: 10 µg/ml. Flow cytometric analysis of Jurkat cells.
  
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide with sequence from the internal region of Human APOBEC3A (NP_663745.1).
• Specificity: This antibody recognizes Human Phorbolin 1 / APOBEC3A.
  Other species not tested.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: APOBEC3A and APOBEC3B deletion hybrid
• Database Link:
  Entrez Gene 100913187 Human
  UniProt ID P31941
• Background: PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.
• Synonyms: Phorbolin-1