Amyloid Precursor Protein (APP) (1-16) Goat Polyclonal Antibody

CAT#: AP31949PU-N

Amyloid Precursor Protein (APP) (1-16) goat polyclonal antibody, Aff - Purified

Product Images

APP antibody staining of Human Cerebral Cortex lysate at 1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:
  Peptide ELISA: 1/8000 (detection limit).
  Western Blot: 1-3 µg/ml. Detects a band of Approx 75kDa in Human Brain (Cerebral and Frontal cortex) lysates and in Mouse adult and fetal Brain lysates.
• Reactivities: Bovine, Canine, Human, Porcine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence DAEFRHDSGYEVHHQK-C, from the N-Terminus of the protein sequence according to NP_000475.1; NP_958816.1; NP_958817.1; NP_001129601.1; NP_001129602.1.
• Specificity:
  This antibody is expected to recognize all reported isoforms of APP (NP_000475.1, NP_958816.1, NP_958817.1, NP_001129601.1, NP_001129602.1) and to recognize all lengths of the Amyloid beta peptide.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 78.7 kDa (NP_958817.1) and 72.6 (NP_001129601.1).
• Gene Name: amyloid beta precursor protein
• Database Link:
  Entrez Gene 351 Human
  UniProt ID P05067
• Background: Amyloid Precursor Protein (APP) ,with 32-amino acid protein (about 35 kDa), is part of a super-family of transmembrane and secreted proteins. It appears to have a number of roles, including regulation of haemostasis and mediation of neuroprotection. APP undergoes alternative splicing, resulting in several isoforms . Proteolytic cleavage of amyloid precursor protein leads to the formation of the 4 kDa amyloid beta/A4 protein, which is present in human platelets. APP is also involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer patients.
• Synonyms: Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebral vascular amyloid peptide, CVAP