NUP84 (N-term) Goat Polyclonal Antibody

CAT#: AP31409PU-N

NUP84 (N-term) goat polyclonal antibody, Aff - Purified

Product Images

NUP84 antibody staining of Saccharomyces cerevisiae S288c lysate at 2 ug/ml (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/8000.
  Western Blot: 2-4 µg/ml. Approx 75kDa band observed in S. cerevisiae wildtype lysates but not in the knock-out strain (calculated MW of 83.6kDa according to NP_010167.1).
• Reactivities: Saccharomyces cerevisiae
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_010167.1.
• Specificity: Recognizes NUP84 (yeast)
• Formulation: Tris saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P52891